FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012142
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA37250.060.704622
CGAGTAG35600.058.4302983
TACTCGG35900.055.3476073
CTACTCG35850.054.0644842
CCCGAGT49750.053.376921
GTAGCTG144500.052.5965086
GCTGGGA294600.052.5244269
AGTAGCT144300.052.1530385
GAGTAGC88950.051.7267954
GCTACTC92800.049.3228871
GGAGGCT226750.046.9637649
CGGAGGT50850.046.524037
TAGCTGG168850.046.2986157
CGTACCG1351.6370905E-1145.994893
TTGGGAG206050.045.895556
CCCAAAG227850.043.8344151
TCGGGAG49250.043.7488446
CTCGGGA45650.043.3903585
AAAGTGC186250.043.2067954
GTGCTGG191600.042.810537
Produced by FastQC (version 0.11.5)