FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012142
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA34900.064.043032
TACTCGG42050.063.784123
CGAGTAG34300.063.7152443
CTACTCG42400.062.6718832
GAGTAGC86200.059.853374
CCCGAGT43950.058.495581
GCTACTC99350.056.2543641
AGTAGCT144400.056.195725
GTAGCTG148050.054.6005946
GCTGGGA302700.054.3535969
TAGCTGG168650.050.6555377
TCGGGAG55400.050.5430686
GGAGGCT248050.050.1845449
CTCGGGA53550.049.854445
TTGGGAG221750.049.1649636
ACTCGGG58650.045.7309844
GTGCTGG191800.044.347267
AAAGTGC195250.044.2314224
AAGTGCT196750.043.8310975
CGGAGGT53650.043.6281137
Produced by FastQC (version 0.11.5)