FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10058784
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG48900.080.389063
CCGAGTA51800.076.531062
GAGTAGC127600.072.6671754
TACTCGG68600.072.571653
CTACTCG71650.068.467082
AGTAGCT212950.068.036445
GCTGGGA499900.068.032979
GCTACTC169200.066.272121
GTAGCTG218750.065.293026
CCCGAGT66700.062.0730741
GGAGGCT403300.061.3327949
AAAGTGC332750.060.2131464
TAGCTGG245700.060.1237957
TCGGGAG89050.059.9607736
AAGTGCT342650.059.7304275
CCCAAAG397550.058.5981031
ACTCGGG86150.057.854054
GTGCTGG350450.057.5960927
CCAAAGT370900.057.5705222
AGTGCTG348050.057.4058046
Produced by FastQC (version 0.11.5)