FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10058784
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46750.076.129113
TACTCGG70800.072.829013
CCGAGTA50300.072.0741652
CTACTCG72850.069.779212
GCTACTC173200.069.125841
GCTGGGA497100.069.038739
GAGTAGC124150.068.971144
AGTAGCT207250.066.738645
GTAGCTG210150.065.395326
GGAGGCT414650.062.234699
AAAGTGC340950.062.1640624
AAGTGCT349300.062.0817765
TCGGGAG90550.061.469286
AGTGCTG354150.060.4528776
CCCAAAG403450.060.3041081
CCAAAGT375650.059.598662
CCCGAGT64650.059.5769351
GTGCTGG354500.059.46997
TAGCTGG240650.058.9721377
CTCGGGA90800.058.3190465
Produced by FastQC (version 0.11.5)