FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11235144
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG101250.080.994283
CGAGTAG61650.080.357043
CTACTCG103150.079.893382
CCGAGTA63350.079.3678362
GCTACTC237000.075.969411
GAGTAGC155000.073.0731354
GCTGGGA617500.071.068459
AGTAGCT245350.070.48225
TCGGGAG126200.069.491936
CCCGAGT78050.068.3949661
CTCGGGA122300.068.3078165
GTAGCTG257500.067.828516
GGAGGCT550200.067.754519
TTGGGAG403000.065.9181446
ACTCGGG123400.065.802344
AAAGTGC413200.064.371744
AAGTGCT418150.063.953985
CCAAAGT449650.063.0851862
CCCAAAG489600.062.7005041
AGTGCTG425250.061.8686526
Produced by FastQC (version 0.11.5)