FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11191558
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG76150.082.3151253
CCGAGTA79900.079.487772
GAGTAGC188200.076.552254
AGTAGCT299850.076.1199345
TACTCGG112750.075.949943
CTACTCG110300.075.569982
GTAGCTG316150.072.8457646
GCTACTC251700.072.600021
GCTGGGA751200.071.5768669
CCCGAGT96450.067.14781
TAGCTGG361850.066.450347
AAGTGCT461850.065.792625
AAAGTGC461150.064.727394
GGAGGCT616100.064.415369
CCAAAGT493350.064.3129962
TTGGGAG457800.064.302716
TCGGGAG146550.063.5475856
CCCAAAG545150.063.0068741
ACTCGGG137500.061.8930324
AGTGCTG486850.061.5012786
Produced by FastQC (version 0.11.5)