FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11191558
Sequences flagged as poor quality0
Sequence length100-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG74700.085.150043
CCGAGTA77800.083.54712
GAGTAGC186550.080.672614
AGTAGCT297200.080.230035
TACTCGG113550.077.804363
CTACTCG112150.076.705342
GTAGCTG314050.076.094336
GCTACTC253150.074.28761
GCTGGGA757300.073.613029
AAGTGCT462750.069.587255
AAAGTGC460150.068.8849644
CCAAAGT485600.068.784722
TAGCTGG360050.068.674287
CCCGAGT97100.067.7693941
TTGGGAG461900.067.13066
CCCAAAG533450.066.9502941
GGAGGCT617050.065.858599
CAAAGTG497700.065.1803743
AGTGCTG488850.064.6236956
ACTCGGG137800.063.342254
Produced by FastQC (version 0.11.5)