FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8790799
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG55850.074.1452262
TACTCGG57450.072.535653
CGAGTAG37350.070.791043
GCTACTC136600.070.4581151
CCGAGTA40150.069.634912
GCTGGGA379500.064.492739
CCCGAGT48350.062.257621
GAGTAGC97900.061.8818634
GGAGGCT333100.061.7478689
AGTAGCT161000.060.6397365
TCGGGAG72500.059.0688936
CTCGGGA70250.058.461485
AAAGTGC258600.057.943074
ACTCGGG71000.057.287174
GTAGCTG170600.056.963476
AAGTGCT269950.056.947325
CCCAAAG313950.056.623931
AGTGCTG271850.055.8631026
GTGCTGG268950.055.8508077
TTGGGAG256100.055.4140176
Produced by FastQC (version 0.11.5)