FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9017338
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG65800.076.030112
TACTCGG68500.073.966033
GCTACTC159400.071.315721
CGAGTAG45200.070.205043
CCGAGTA47150.069.149272
GAGTAGC113900.067.8825764
AGTAGCT186700.067.349285
GCTGGGA453950.066.0971769
GTAGCTG195950.064.1593256
GGAGGCT375650.062.034779
TCGGGAG87100.060.9620976
CTCGGGA84650.060.009255
ACTCGGG84800.058.9695474
TAGCTGG224800.058.5071077
AAGTGCT302250.058.1899875
AAAGTGC299900.057.5460784
TTGGGAG283050.056.743746
CCCAAAG355500.056.405461
CCCGAGT58250.056.2769971
AGTGCTG307500.056.028666
Produced by FastQC (version 0.11.5)