FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9017338
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG47750.077.230693
TACTCGG65000.075.308163
CTACTCG66600.074.489512
CCGAGTA50400.072.515482
GCTACTC157450.071.539111
GAGTAGC118350.070.569484
AGTAGCT190150.069.2385945
GCTGGGA450150.067.9700249
GTAGCTG198950.065.073996
GGAGGCT373000.063.303039
TCGGGAG85700.061.6428536
AAAGTGC294300.061.4875224
CCCGAGT61300.061.0344771
AAGTGCT300100.060.7733545
CTCGGGA84050.060.1139265
TAGCTGG220700.060.0970047
CCCAAAG347500.059.8147661
ACTCGGG83300.059.3182454
CCAAAGT322600.059.2632522
TTGGGAG288400.059.046216
Produced by FastQC (version 0.11.5)