FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18825280
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG104750.082.908523
TACTCGG169100.080.0270163
CCGAGTA113100.079.666782
CTACTCG166000.079.228142
GAGTAGC267700.076.6893544
GCTACTC391300.075.1394961
AGTAGCT443650.074.4289865
GCTGGGA1129500.074.1972669
GTAGCTG459850.071.50286
TCGGGAG213700.068.023736
GGAGGCT932200.067.631899
CTCGGGA208500.066.649455
CCCGAGT140450.066.4562151
AAGTGCT791100.065.677515
AAAGTGC774800.065.486924
ACTCGGG206550.065.200834
CCAAAGT834600.064.526852
CCCAAAG903450.064.336261
TAGCTGG527200.064.310367
AGTGCTG806700.063.5766
Produced by FastQC (version 0.11.5)