FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834950
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG19150.048.102573
CCGAGTA20700.046.5440832
TACTCGG12200.045.842483
AGTAGCT72000.045.3662955
GAGTAGC45150.043.823334
CTACTCG12900.043.7192272
GTAGCTG73400.043.4765136
CCCGAGT22900.043.177011
TAGCTGG78400.042.6220667
TTGGGAG100450.040.4245156
GCTACTC32650.040.0894781
GCTGGGA131450.040.0088589
AAAGTGC60100.038.083384
GCACTTT84950.037.3564261
CCCAAAG78050.036.7981261
CCAAAGT69200.036.3352932
AAGTGCT64250.035.9161155
ACTTTGG88750.035.3214453
CAAAGTG68600.034.9403343
TTTGGGA100350.034.938355
Produced by FastQC (version 0.11.5)