FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3834950
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT66600.048.2526635
CGAGTAG17750.047.6439673
CCGAGTA18700.045.9772762
GTAGCTG71000.045.262366
GCTACTC38500.043.9371831
CTACTCG17350.043.868152
GAGTAGC42600.043.343344
GCTGGGA132800.043.268669
TACTCGG17450.042.8090363
CCCGAGT21650.042.756171
TAGCTGG79450.042.695567
TTGGGAG112400.041.8832936
GCACTTT88300.040.6026341
AAAGTGC62550.040.0349544
CCCAAAG79750.039.770721
CCAAAGT72050.039.0594942
TTTGGGA104350.038.7208985
ACTTTGG91800.038.6912573
GGAGGCT107400.038.5841949
AAGTGCT66250.038.579135
Produced by FastQC (version 0.11.5)