FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397159
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG10050.046.2799643
CTACTCG8250.043.8490942
TACTCGG8600.043.703423
AGTAGCT39050.043.4311145
CCGAGTA10500.043.4016532
GAGTAGC23950.042.9589234
GCTGGGA71850.040.4733929
GTAGCTG40200.040.3179866
TAGCTGG43500.039.851337
TTGGGAG58050.039.0077176
AAAGTGC35850.038.0035064
GCTACTC20400.037.36471
GCACTTT50000.036.3240241
CCCGAGT14250.036.3207171
CCCAAAG48150.034.3971941
ACTTTGG49050.034.385813
TTTGGGA54000.034.191145
GGAGGCT58250.033.9540759
AAGTGCT40350.033.4158975
CCGGGTT6800.033.1617978
Produced by FastQC (version 0.11.5)