FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3179766
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13150.036.5270422
CGGAGGT12600.035.43167
AGTAGCT46050.035.0711335
CGAGTAG13150.034.3740583
GTAGCTG48000.034.3688556
GCTGGGA83650.033.9319429
CCCGAGT16150.033.8277741
GAGTAGC29000.033.2844354
TTGGGAG68700.032.5648736
CCCGAAT4500.032.4442061
TAGCTGG55650.031.4978837
CGATACG600.004122759731.3941572
TCGCGAA753.2791932E-431.32948794
CTACTCG8950.030.5172252
GGAGGTT35250.029.9958468
TACTCGG9150.029.3318273
GGCGGAG18300.029.074535
AGCGCGT650.00615994728.9190738
AGGCGGA17350.028.766814
GCACTTT57800.028.6815621
Produced by FastQC (version 0.11.5)