FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867969
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA18050.036.7136232
TTGGGAG81100.035.814016
AGTAGCT60550.035.627385
CGAGTAG17500.035.4504053
GAGTAGC40600.034.2650224
GTAGCTG62150.034.256456
GCTGGGA108900.033.7065359
CCCGAGT22100.033.4556431
TAGCTGG67300.032.333397
CTACTCG12300.032.0966382
GGAGGCT82650.032.015229
TACTCGG12200.031.9744853
GCTACTC31000.031.5982931
GCACTTT71000.030.4450241
TTTGGGA82950.030.4258845
GGGAGGC111000.029.7658088
ACTTTGG75950.028.836563
TGGGAGG114650.028.1212987
CACTTTG78950.028.0384562
ATGCGCG1354.6406603E-727.8510464
Produced by FastQC (version 0.11.5)