FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764492

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764492
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3867969
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTG60350.038.458836
TTGGGAG89450.038.23826
GAGTAGC38200.038.1285554
CGAGTAG15450.038.0131263
AGTAGCT59800.037.870225
GCTGGGA112750.037.7545479
CCGAGTA16250.037.2977562
CTACTCG14000.035.5733032
TAGCTGG67100.034.5195587
TACTCGG14650.034.316123
GGAGGCT92850.034.1569829
CCCGAGT20700.033.369921
AAAGTGC60550.032.047044
GCACTTT74600.031.9987531
GGGAGGC122200.031.7970148
TTTGGGA86800.031.7197635
CCCAAAG75550.031.4097941
CCAAAGT70900.030.4830232
ACTTTGG79400.030.415483
AAGTGCT64650.030.3053675
Produced by FastQC (version 0.11.5)