FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2951663
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG9300.040.4115032
TACTCGG9400.039.4818233
CGAGTAG11300.037.832143
AGTAGCT40550.037.3027575
CCGAGTA11750.037.1828842
TTGGGAG61650.036.0415956
GAGTAGC25200.035.606024
CGGAGGT13100.034.783757
GCTGGGA76350.034.701329
GTAGCTG43300.034.2827036
GGAGGCT59300.033.429799
TAGCTGG46850.032.6876687
CCCGAGT14750.032.5264131
GCTACTC25000.032.1724471
TCGGGAG12700.030.7008426
CTCGGGA12450.030.562695
GGGAGGC83800.030.2709228
GCACTTT55250.029.2004721
TTTGGGA63900.029.1118815
AGGCGGA20050.027.6476524
Produced by FastQC (version 0.11.5)