FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504512
Sequences flagged as poor quality0
Sequence length100-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG69800.076.835613
CCGAGTA73250.075.091622
AGTAGCT255150.071.949865
GAGTAGC163850.070.6160664
GTAGCTG261400.069.9417346
CTACTCG76900.066.328512
GCTGGGA605000.063.4956749
TACTCGG81150.062.8322533
TAGCTGG301750.062.7248157
GCTACTC184100.062.1840821
CCCGAGT90750.061.2605321
TTGGGAG386650.059.7855226
AAGTGCT329750.058.8197145
AAAGTGC330950.057.7104874
GCACTTT267050.056.9164921
GGAGGCT469000.056.611519
ACTTTGG275650.056.217453
CCAAAGT360350.056.0642432
CCCAAAG400750.054.5876161
CAAAGTG359000.054.154123
Produced by FastQC (version 0.11.5)