FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9504512
Sequences flagged as poor quality0
Sequence length100-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG65650.079.53333
CCGAGTA71200.075.05392
AGTAGCT249750.072.519345
GAGTAGC161100.071.223484
CTACTCG83900.069.916252
TACTCGG85100.069.0818563
GTAGCTG263550.068.313176
GCTACTC185300.065.973081
TTGGGAG391000.063.795126
GCTGGGA610700.063.0064969
TAGCTGG299250.062.0382167
GCACTTT273800.062.0042271
AAGTGCT327950.061.1231585
CCCGAGT89800.060.9586371
AAAGTGC335300.059.212844
ACTTTGG290550.058.9924163
CCAAAGT354900.058.9396322
CACTTTG290500.058.203742
GGAGGCT484950.058.1229979
TTTGGGA331950.057.8235865
Produced by FastQC (version 0.11.5)