FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764497

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764497
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763264
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1156.184564E-1140.906055
CGCGTTG1251.5097612E-1037.6001976
CTCGCGT1401.2732926E-1136.9615364
TACTCGG8950.034.6920433
CTACTCG8350.034.3697172
TTGGGAG56500.033.108146
GCTGGGA63400.032.7637679
GGAGGCT52550.032.6423159
GCTACTC20650.031.249621
CCGAGTA9650.031.2022042
GGGAGGC67000.031.0751538
AGTAGCT34900.031.0018635
GTAGCTG35600.029.9692556
GCACTTT48300.029.7438351
CGAGTAG9050.029.6302453
CCCGAGT12800.029.4390811
CGGAGGT9500.028.6938487
CTCGGGA10450.028.3602265
GAGTAGC22900.028.3484294
TTTGGGA59450.027.6950135
Produced by FastQC (version 0.11.5)