FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764500

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764500
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11953508
Sequences flagged as poor quality0
Sequence length100-150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG66650.078.971593
CCGAGTA71200.075.606232
TACTCGG90100.074.2963943
CTACTCG91500.072.795092
GAGTAGC163900.072.671344
AGTAGCT266100.070.466325
GCTGGGA638700.070.273559
GTAGCTG275350.067.359736
CCCGAGT85900.066.326081
GGAGGCT513150.062.5505459
TAGCTGG307300.062.4095577
TCGGGAG116800.062.0265586
AAAGTGC422650.061.8571974
CCAAAGT455250.061.5494772
AAGTGCT435750.061.4324845
ACTCGGG110300.061.4131434
CCCAAAG500700.060.935551
CTCGGGA114000.060.2365765
AGTGCTG440200.060.0277256
TTGGGAG394500.058.9882436
Produced by FastQC (version 0.11.5)