FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9928848
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG58600.086.015363
TACTCGG98050.083.227843
CTACTCG98200.082.071392
CCGAGTA63250.081.929042
GCTACTC222500.079.026941
GAGTAGC143450.078.918864
AGTAGCT232400.076.683995
GCTGGGA603000.075.11269
GTAGCTG246450.072.138436
AAGTGCT392950.071.827095
TCGGGAG121250.071.341036
GGAGGCT524250.070.8076559
AAAGTGC394100.070.070884
CTCGGGA119250.068.818325
TTGGGAG382000.068.51476
ACTCGGG117550.068.504224
CCAAAGT425100.068.2899252
CCCGAGT77300.068.1802141
CCCAAAG463150.067.781141
AGTGCTG412650.066.543936
Produced by FastQC (version 0.11.5)