FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601024
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52100.082.385683
CCGAGTA56950.077.3639762
CTACTCG68550.073.3182452
TACTCGG71100.073.122713
GAGTAGC132850.072.867844
AGTAGCT211150.071.488445
GCTACTC165050.068.450371
GTAGCTG218750.068.438146
GCTGGGA499050.068.2588359
CCCGAGT67250.067.431611
TAGCTGG250700.062.5783777
GGAGGCT399300.062.488239
AAAGTGC305050.061.3084374
TTGGGAG310450.060.6412626
AAGTGCT313950.060.422335
TCGGGAG92800.059.7495356
CCAAAGT333600.059.695962
CCCAAAG368750.058.5469631
CTACTTG110850.058.3534742
CTCGGGA89850.058.2989925
Produced by FastQC (version 0.11.5)