FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8601024
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG52650.077.430693
TACTCGG68200.076.608963
CTACTCG68900.075.073292
CCGAGTA56300.073.569332
GAGTAGC126350.073.564984
GCTACTC160500.072.471691
AGTAGCT207100.072.094095
GCTGGGA485300.069.367349
GTAGCTG214950.069.006036
AAGTGCT305450.064.811585
AAAGTGC305050.064.575444
GGAGGCT401450.064.082129
TTGGGAG318950.063.8271066
CCAAAGT326400.063.1692662
TAGCTGG243000.062.837897
CCCGAGT68850.062.6310351
CCCAAAG359150.062.3213541
AGTGCTG313400.061.648156
TCGGGAG92100.061.1202556
CTCGGGA88800.060.6003275
Produced by FastQC (version 0.11.5)