FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3929059
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1051.1423253E-940.2774165
TACGCGG757.4587224E-637.592265
TACTCGG12650.035.6601873
TTGGGAG74250.033.6680566
CGAGTAG14650.033.357893
CCGAGTA15400.032.64872
GAGTAGC35300.031.9480934
AGTAGCT54500.031.9016845
GGAGGCT79150.031.464619
CTACTCG13050.031.326482
GTAGCTG54600.030.9822356
GCTACTC33450.030.9070151
CGGAGGT15950.030.6382877
GCTGGGA90650.030.5312639
CGCCGTA1751.70985E-1029.54767894
GGGAGGC102800.028.7507618
TAGCTGG59350.028.1060267
CCGGGTT11100.027.9391238
CCCGAAT6950.027.7223191
GCACTTT65200.027.3883911
Produced by FastQC (version 0.11.5)