FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764509

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764509
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8680166
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG39350.060.305373
CCGAGTA42800.058.473452
TACTCGG47500.057.5549743
CTACTCG48450.056.832652
GCTACTC115100.055.9867631
AGTAGCT165350.055.741425
GTAGCTG166650.055.4533926
GAGTAGC105550.054.8732574
GCTGGGA352900.054.8348059
TTGGGAG264750.053.571496
GGAGGCT292000.051.7351349
TAGCTGG192900.049.8095867
CCCGAGT55150.049.648591
AAGTGCT212300.049.2623445
AAAGTGC209000.049.162274
TCGGGAG63150.047.4560626
CTCGGGA60700.046.7517935
CCCAAAG270200.046.2931
AGTGCTG222900.045.8221446
CCAAAGT238400.045.738362
Produced by FastQC (version 0.11.5)