FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8680166
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG49350.066.9164352
TACTCGG50450.065.82223
CGAGTAG42200.063.3290143
GCTACTC122800.060.4629171
GAGTAGC104900.059.581194
CCGAGTA44650.059.4421542
AGTAGCT169200.058.5890435
GCTGGGA361850.057.9522329
GTAGCTG169550.057.7834476
TTGGGAG271400.056.806996
GGAGGCT306650.055.999669
CCCGAGT52850.054.3903241
TAGCTGG189350.054.001367
TCGGGAG66950.052.2497446
AAGTGCT223100.051.670845
CTCGGGA64300.051.6443255
GCACTTT202700.051.5954171
AAAGTGC224300.051.1490174
CCCAAAG277150.049.5195581
CCAAAGT252250.049.0688132
Produced by FastQC (version 0.11.5)