FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10668889
Sequences flagged as poor quality0
Sequence length100-150
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG55200.074.053233
TACTCGG77850.072.936723
CCGAGTA57550.072.472122
CTACTCG76500.070.5502242
GAGTAGC139100.070.023834
AGTAGCT224600.067.79535
GCTACTC177200.065.863861
GCTGGGA515100.065.3824549
GTAGCTG233750.064.595026
CCCGAGT70250.064.108431
GGAGGCT428900.060.325539
TCGGGAG99550.059.8609356
CTCGGGA96150.059.8519945
TTGGGAG347750.059.5638776
TAGCTGG264350.058.6627357
ACTCGGG95500.058.6542324
AAGTGCT333450.058.6321375
AAAGTGC334000.057.981024
CCCAAAG399950.056.509891
CCAAAGT364250.056.0415732
Produced by FastQC (version 0.11.5)