FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764513

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764513
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTAGCT49450.042.198665
TACTCGG9600.041.613063
CCGAGTA14400.041.449872
GAGTAGC31900.041.399744
CGAGTAG14050.040.8098953
GTAGCTG49650.040.6087886
TTGGGAG74850.038.992566
CTACTCG9700.038.276952
TAGCTGG54900.037.667117
CCCGAGT16600.037.4486731
GCTGGGA92150.037.334029
GCACTTT62800.037.195691
GCTACTC23450.036.550991
GGAGGCT69050.034.5771379
CACTTTG66950.034.3273472
GGGAGGC94950.034.153578
CCCAAAG61400.033.9019361
AAAGTGC48000.033.8779264
TTTGGGA74550.033.790855
ACTTTGG68150.033.3091243
Produced by FastQC (version 0.11.5)