FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3082198
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA13550.043.692952
GCTACTC26300.042.707071
TTGGGAG85050.042.5965356
CTACTCG11250.042.184122
TACTCGG11650.041.5423853
GTAGCTG49650.041.3577046
AGTAGCT49450.040.9535065
CGAGTAG13900.040.902583
GAGTAGC31800.040.338124
GCTGGGA90950.040.194929
CGGAGGT17000.039.8009727
GCACTTT68650.039.4311641
CCCGAGT16200.039.163041
TTTGGGA81050.038.146355
ACTTTGG71750.037.9827353
CACTTTG71800.037.7599562
GGAGGCT74450.037.679399
TAGCTGG55850.037.017727
CTTTGGG81900.035.3408474
GGGAGGC110550.035.3212858
Produced by FastQC (version 0.11.5)