FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117869
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG14450.045.31883
CCGAGTA15050.044.7699782
CCCGAGT18250.040.7972071
AGTAGCT50200.040.166555
GTAGCTG51750.038.3303836
GAGTAGC34300.037.9093554
GCTGGGA91950.036.898459
TTGGGAG73900.036.5733266
CTACTCG10650.036.2786872
TAGCTGG55350.035.318187
TACTCGG11550.034.671163
GCTACTC26400.033.3790441
CCCAAAG59850.032.8328131
AAAGTGC44700.032.567354
CGGAGGT13000.032.171357
GGAGGCT67900.031.9046369
GCACTTT62000.031.3902361
TTTGGGA74650.030.8605385
CCAAAGT55550.030.620372
AAGTGCT47150.030.5748395
Produced by FastQC (version 0.11.5)