FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3117869
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN89530.28715125619453546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA14600.047.3279382
CGAGTAG14200.047.0044633
AGTAGCT49850.043.6542855
CTACTCG11900.043.4509052
GAGTAGC33500.042.9347274
TACTCGG12400.042.076583
CCCGAGT17850.041.6337471
CGGAGGT17250.041.3796847
GTAGCTG52000.041.1913726
GCTGGGA96650.039.4491659
TTGGGAG78800.039.4021576
TAGCTGG57650.038.3666847
GCACTTT64900.036.6718181
AAAGTGC50000.035.7228134
CCCAAAG64200.035.2400631
GGAGGCT79800.035.1281369
TTTGGGA76950.034.510475
CCGTACG550.002707647934.2077061
CGTACCG550.00271645134.1850663
GGAGGTT44800.034.1688428
Produced by FastQC (version 0.11.5)