FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714757
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT450.001011491541.800495
AGTAGCT38250.038.2351535
GAGTAGC24900.037.7715264
CCGAGTA10650.037.0960462
GTAGCTG39400.036.7394266
CGAGTAG10000.036.6826363
GCTGGGA70900.034.6014379
TTGGGAG54550.034.4622086
CCCGAGT14150.033.274691
TAGCTGG44900.032.6577387
GGAGGCT51900.030.96919
GGGAGGC69200.029.0680268
GCTACTC18750.028.3757691
CTACTCG7500.028.219492
GCACTTT50450.028.1848851
CCCAAAG45750.027.5812641
CTCGCGT1205.8223704E-627.4315724
CGGAGGT9800.027.3354767
TACTCGG7750.027.3071733
TGGGAGG72850.027.2245547
Produced by FastQC (version 0.11.5)