FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2714757
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA10350.042.703332
CGAGTAG9850.041.0522083
CTACTCG9750.039.5442662
TACTCGG9950.039.221963
TCGCGTT853.9468614E-738.720185
TCGCGAA500.001702275437.6007294
GAGTAGC23250.036.8056644
TTGGGAG60150.036.7955636
CCCGAGT13550.035.7724381
GCTACTC21750.035.7005841
AGTAGCT37200.035.0102425
GGAGGCT57600.034.755329
GTAGCTG36400.034.726516
GCTGGGA69750.033.9563149
TAGCTGG40750.032.7504237
TCGGGAG13550.032.251796
GGGAGGC81300.031.9645658
GCACTTT52550.031.5224951
CTCGGGA12750.031.344915
ACTTTGG52200.031.0758383
Produced by FastQC (version 0.11.5)