FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8608943
Sequences flagged as poor quality0
Sequence length100-150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA34900.060.7823832
TACTCGG41850.060.7304083
CGAGTAG34300.060.7114873
CTACTCG41450.060.383152
GCTGGGA312050.057.384019
GAGTAGC86700.056.0831154
GCTACTC106400.053.6078451
CCCGAGT45950.053.4007951
AGTAGCT147300.053.0700345
GGAGGCT253650.052.1253939
TCGGGAG53500.051.579296
GTAGCTG149550.051.553166
CTCGGGA51750.049.852665
CGGAGGT54850.049.364647
TAGCTGG167850.048.355617
AAAGTGC222900.048.1829034
GTGCTGG215300.048.023587
CCCAAAG271850.047.488651
TTGGGAG219900.047.3447236
AAGTGCT229500.047.0218245
Produced by FastQC (version 0.11.5)