FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764521

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764521
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7940833
Sequences flagged as poor quality0
Sequence length100-150
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG46850.079.553273
CCGAGTA49150.077.056742
TACTCGG61950.074.183053
CTACTCG63400.072.7280042
GAGTAGC116500.071.4395754
AGTAGCT192550.070.7938465
GTAGCTG194750.069.328476
GCTACTC149400.068.594971
GCTGGGA452350.068.178849
CCCGAGT60750.062.8191071
GGAGGCT366550.062.793879
TAGCTGG227150.061.7510457
AAAGTGC284600.061.356774
AAGTGCT289900.061.1070335
TTGGGAG289350.060.954376
CCAAAGT307650.060.561982
TCGGGAG78700.059.794566
CCCAAAG341150.059.1474571
AGTGCTG299600.058.306476
CTCGGGA78250.057.9018755
Produced by FastQC (version 0.11.5)