FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034185
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG13500.043.9536553
CCGAGTA13600.041.9060172
AGTAGCT52750.040.7992255
GAGTAGC35000.039.4237334
CCCGAGT16000.038.8653681
GTAGCTG54800.038.0834776
GCTGGGA98150.037.5842749
TAGCTGG59000.036.797377
TACTCGG8550.036.3526463
TTGGGAG77150.036.128756
GGAGGCT67350.035.444839
CTACTCG8400.034.204282
GCACTTT63350.034.1330341
CCCAAAG59800.032.6930731
AAGTGCT48300.032.4679835
AAAGTGC47900.032.2475364
GCTACTC23200.032.083221
ACTTTGG66200.032.0119933
CACTTTG68800.031.6973152
GGGAGGC96000.031.426118
Produced by FastQC (version 0.11.5)