FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3034185
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN86140.28389831206732613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTCG11350.043.910742
AGTAGCT52950.043.681975
GTAGCTG53550.043.5112156
TACTCGG11350.043.494323
CGAGTAG12800.042.974913
CCGAGTA12700.042.20482
TTGGGAG79250.041.19696
GAGTAGC33900.041.1890224
TAGCTGG58750.040.928517
GCTGGGA99850.040.632359
GCTACTC28850.036.2129861
GCACTTT62800.036.1197361
CCCAAAG64700.035.9318731
GGAGGCT78400.035.8171779
CCAAAGT57950.035.4559142
TTTGGGA75250.035.360065
AAAGTGC52000.035.2602964
AAGTGCT52700.035.0578275
CCCGAGT16300.034.645821
GGGAGGC111600.034.3351868
Produced by FastQC (version 0.11.5)