FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764528

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764528
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3460452
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12150.041.3761062
CGGAGGT12950.039.545097
TACTCGG12300.038.5796623
AGTAGCT47150.037.5665935
TTGGGAG74250.037.3334126
CGAGTAG12500.036.834793
CTACTCG12650.035.6551932
GTAGCTG48350.035.2738046
GCTGGGA85650.035.051729
CCCGAGT17200.034.6930541
CTCGGGA13800.034.386225
GAGTAGC31900.033.5804334
GCACTTT63250.033.5029641
GCTACTC30250.033.239591
GGAGGCT73100.033.1640979
GGGAGGC97100.032.515168
TAGCTGG54500.032.4135937
TCGGGAG15600.031.0209276
GGAGGTT40150.031.0096028
TTTGGGA73000.030.699955
Produced by FastQC (version 0.11.5)