FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882734
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA21000.051.45552
CGAGTAG20650.051.1907353
CTACTCG14600.048.5900572
AGTAGCT73450.048.2929845
CCCGAGT23800.047.9767651
GTAGCTG73300.047.7514726
GAGTAGC48550.046.4494824
TACTCGG15300.046.3675773
TAGCTGG80400.044.7033277
GCTGGGA136850.044.5276379
TTGGGAG107300.042.2096566
GCTACTC37000.042.1636281
GCACTTT88450.040.109821
AAGTGCT68400.039.9069865
AAAGTGC68500.039.437214
CCCAAAG82400.039.119971
CCAAAGT77000.038.378082
ACTTTGG91400.038.140523
GGAGGCT102400.038.127269
TTTGGGA103750.038.0834965
Produced by FastQC (version 0.11.5)