FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8486788
Sequences flagged as poor quality0
Sequence length100-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG79500.082.144023
CTACTCG78050.082.081932
CGAGTAG54800.080.715723
GAGTAGC135950.080.043454
AGTAGCT214500.079.10835
GCTACTC180150.078.305881
CCGAGTA58450.076.568232
GTAGCTG225750.075.2527166
GCTGGGA541950.073.3467569
AAGTGCT335000.070.068915
AAAGTGC338950.068.520944
TAGCTGG256750.067.970797
GGAGGCT446000.067.360269
TTGGGAG346400.067.29216
CCAAAGT360200.067.2859042
CTCGGGA99700.066.744285
TCGGGAG104550.066.580866
CCCAAAG392150.066.388621
CTACTTG118850.066.199872
CCCGAGT69600.066.09261
Produced by FastQC (version 0.11.5)