FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2871891
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT503.0061485E-547.011675
CGAGTAG11000.038.4640963
CCGAGTA11400.038.35632
AGTAGCT44650.034.1137285
GAGTAGC28450.033.8748444
CTACTCG7700.033.583862
GCTGGGA77100.033.5837339
GTAGCTG46350.032.963956
CTCGCGT1309.1768015E-932.5465434
CCCGAGT15250.032.4580151
TAGCTGG49950.031.994927
TACTCGG7600.031.5473063
TTGGGAG60000.031.497826
GGGAGGC72550.028.8887778
GCTACTC19500.027.801341
CGGAGGT11350.027.7470327
GGAGGCT54700.027.6630639
GCACTTT48550.027.3818231
TGGGAGG76750.026.4571447
TTTGGGA62700.026.3175375
Produced by FastQC (version 0.11.5)