FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4047879
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGTT1450.038.895535
CCGAGTA16600.035.9568862
GTAGCTG59350.035.5559356
AGTAGCT59450.034.8636785
TTGGGAG90600.033.9781346
CGAGTAG16800.033.5705453
GCTGGGA108050.033.1018079
CCCGAGT20650.032.605971
TAGCTGG66100.032.138347
GGAGGCT83100.032.011599
GAGTAGC40950.031.1029664
GCTACTC31650.031.0923611
CTACTCG11950.031.0703142
GCACTTT75900.030.7075021
TACTCGG12800.030.4757633
CGGAGGT16400.030.0906727
CGTACGT953.7552614E-529.683432
CCCAAAG71500.029.3704051
GGGAGGC116900.028.9872638
ACTTTGG80400.028.4681573
Produced by FastQC (version 0.11.5)