FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9417565
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG42500.071.780793
CCGAGTA44450.071.1217962
TACTCGG49850.065.372773
CTACTCG52150.062.6163332
GAGTAGC108850.061.002534
CCCGAGT56350.060.5484051
GCTACTC128900.060.515861
GCTGGGA399400.060.3598189
AGTAGCT184100.059.5246245
GTAGCTG183800.059.1657456
GGAGGCT310700.056.065119
TAGCTGG205400.055.0298427
TCGGGAG67650.053.3267446
CTCGGGA67250.051.158045
AAAGTGC270650.050.701994
CCCAAAG328400.049.806021
GTGCTGG275400.049.562657
ACTCGGG65950.049.5039024
AAGTGCT280900.049.4306875
AGTGCTG277300.049.342616
Produced by FastQC (version 0.11.5)