FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9417565
Sequences flagged as poor quality0
Sequence length100-150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG55600.071.117973
CGAGTAG42600.070.836813
CTACTCG57250.067.932292
CCGAGTA45300.067.1892
GAGTAGC110750.066.532944
GCTGGGA410200.064.521369
GCTACTC134750.063.630011
AGTAGCT188100.063.548115
GTAGCTG190000.062.8623476
CCCGAGT53750.060.6365621
TAGCTGG207900.060.197577
TCGGGAG70100.059.1749236
GGAGGCT321000.058.4911659
CTCGGGA72600.055.8976945
AAAGTGC271600.055.289764
ACTCGGG73050.054.2185134
GTGCTGG278250.053.9032757
AAGTGCT282050.053.701865
AGTGCTG281550.053.4140056
CCCAAAG321300.052.5819321
Produced by FastQC (version 0.11.5)