FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431170
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA12500.035.4380652
CCCGAGT16200.034.3322031
GCTGGGA82800.032.5192579
CGGAGGT12150.032.487737
AGTAGCT45050.032.3200075
CGAGTAG12700.031.908213
CGTACGA300.00416623631.3274572-73
TACTCGG9700.030.118113
TTGGGAG65700.029.9785446
GTAGCTG48100.029.9046176
GAGTAGC30250.029.1288914
TAGCTGG51750.028.6941327
CTACTCG9400.028.5758512
CGGGTTC8200.027.507039
TCGCGTT1205.7325797E-627.4868225
GGGAGGC81350.027.26478
GGCGGAG16600.027.2502775
GGAGGCT60150.026.9525479
GGAGGTT33050.026.730238
CCGGGTT8100.026.6863488
Produced by FastQC (version 0.11.5)