FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3431170
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN98380.2867243535004095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTCGG10400.037.5256923
CTACTCG10450.036.8951032
CGGAGGT14950.035.183447
CCGAGTA12750.035.033592
AGTAGCT43550.033.7920575
CGAGTAG11800.033.4719623
CCCGAGT16000.032.64611
TTGGGAG71100.032.377316
GCTGGGA84000.032.309289
GAGTAGC29400.032.145954
GCTACTC27150.031.3716261
GTAGCTG46300.031.150986
GGAGGCT67850.031.072439
GGAGGTT38300.030.8958118
GGCGGAG23300.029.6633245
GCGGAGG19100.028.5324426
TAGCTGG50850.028.4460037
CTCGGGA14600.028.3392415
AGGCGGA23050.027.9464864
GGGAGGC93600.027.7425968
Produced by FastQC (version 0.11.5)