FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3250031
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAGTA11550.035.8009952
TACTCGG9800.033.0839543
TTGGGAG64950.033.061676
CTACTCG9400.032.9921382
CGAGTAG11500.031.8706673
CGCAACG902.5998732E-531.3253886
TCGCGTT902.5998732E-531.3253885
AGTAGCT42900.030.7777425
GTAGCTG42100.030.2464836
GGAGGCT60000.030.2285359
GGGAGGC79350.030.1405988
GCTGGGA77150.029.4774889
CTCGGGA11450.029.1367155
TAGCTGG48500.028.3861777
CCGGGTT7550.028.0057098
CGGAGGT12450.027.9282287
GAGTAGC27500.027.8515454
CTCGCGT1354.648773E-727.8452194
CCCGAGT16850.027.6119161
TGGGAGG80900.027.5883777
Produced by FastQC (version 0.11.5)