FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005764549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005764549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4009358
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTAG18150.042.2081383
AGTAGCT71150.041.2187585
CCGAGTA18850.040.640732
GAGTAGC45450.040.2254524
GTAGCTG72450.039.0520066
GCTGGGA131000.038.856059
TTGGGAG101400.038.5166326
TAGCTGG79800.037.8698237
TACTCGG13900.036.5169223
CTACTCG14100.036.3322752
AAAGTGC64050.035.4415934
CCCGAGT24100.035.359021
GGAGGCT92650.035.2059069
GCACTTT84500.034.9898151
GCTACTC32350.034.7826231
AAGTGCT67900.033.639675
CGGAGGT18600.033.6065527
TTTGGGA98150.033.5191465
ACTTTGG88000.033.1126753
CCAAAGT73650.032.9915732
Produced by FastQC (version 0.11.5)